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    • Keywords


      Phage φ149; phage coded tRNAs; tRNA-gene cloning

    • Abstract


      Vibrio cholerae phage φ l49 codes tRNAs specific for twelve different amino acids. These tRNA genes are contained in two different HindIII fragments 11 and 3.4 kb in size, of the phage genome. The 3.4 kb HindIII fragment was cloned inEscherichia coli using pBR328 as vector. The recombinant plasmid pNR347 produced nine of the twelve tRNA species (arginine, proline, serine, tyrosine, histidine, lysine, leucine, tryptophan and aspartic acid) encoded in the phage genome.

    • Author Affiliations


      Nripendranath Manual1 Ranajit Kumar Ghosh1

      1. Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, Jadavpur, Calcutta - 700 032, India
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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