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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/017/02/0151-0165

    • Keywords

       

      Riboflavin carrier protein; antigenic determinants; monoclonal antibodies

    • Abstract

       

      Monoclonal antibodies raised against chicken egg white riboflavin carrier protein were classified into seven categories each recognizing a distinct epitope. Of these, six were directed against conformation dependent epitopes and one to a sequential epitope. The roles of lysine residues and the post-translationally attached phosphate and oligosaccharide moieties in the antigenicity of riboflavin carrier protein recognized by the monoclonal antibodies were investigated. The binding region of three monoclonal antibodies could be located within the 87–219 amino acid sequence of the protein and one antibody among these recognized a sequence of 182–204 amino acid residues. All the monoclonal antibodies were able to recognize riboflavin carrier proteins present in the sera of pregnant rats, cows and humans indicating that the epitopes to which they are directed are conserved through evolution from chicken to the human.

    • Author Affiliations

       

      N Kuzhandhai Velu1 Anjali A Karande1 P R Adiga1

      1. Centre for Reproductive Biology and Molecular Endocrinology and Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012, India
    • Dates

       
  • Journal of Biosciences | News

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