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      https://www.ias.ac.in/article/fulltext/jbsc/017/01/0055-0066

    • Keywords

       

      Mini-exon derived RNA; Leishmania donovani ; cloning; organization; promoter elements

    • Abstract

       

      Mini-exon derived RNA is a small nuclear RNA of trypanosomatid protozoa such asLeishmania which donates its 5′-terminal 39 nucleotides to the 5’-ends of cellular messenger RNAs by trans-splicing. We have cloned a mini-exon derived RNA gene fromLeishmania donovani and studied its organization and expression. About 200 copies of the gene per haploid genome are organized as a tandem repeat on a single chromosome. The gene is transcribed as a 95-nucleotide RNA. The first 39 nucleotides of mini-exon derived RNA is also found at the 5′-terminus of a cellular mRNA (Β-tubulin), thus confirming its identity. Sequence analysis of the gene and its flanking regions showed that while classical RNA polymerase II promoter elements such as TATA and CAAT are absent from the 5′-upstream region, intragenic sequence motifs resembling RNA polymerase III promoter elements are present. The implications of this finding for mini-exon derived RNA expression are discussed.

    • Author Affiliations

       

      Md Quamarul Hassan1 Saumitra Das1 Samit Adhya1

      1. Genetic Engineering Laboratory (Leishmania Group), Indian Institute of Chemical Biology, 4 Raja S C Mullick Road, Calcutta - 700 032, India
    • Dates

       
  • Journal of Biosciences | News

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