• Restriction enzyme digestion of heterochromatin inDrosophila nasuta

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      https://www.ias.ac.in/article/fulltext/jbsc/016/04/0187-0197

    • Keywords

       

      Drosophila ; heterochromatin: restriction enzyme digestion

    • Abstract

       

      In situ digestion of metaphase and polytene chromosomes and of interphase nuclei in different cell types ofDrosophila nasuta with restriction enzymes revealed that enzymes like AluI, EcoRI, HaeIII, Sau3a and SinI did not affect Giemsa-stainability of heterochromatin while that of euchromatin was significantly reduced; TaqI and SalI digested both heterochromatin and euchromatin in mitotic chromosomes. Digestion of genomic DNA with AluI, EcoRI, HaeIII, Sau3a and KpnI left a 23 kb DNA band undigested in agarose gels while withTaqI, no such undigested band was seen. TheAluI resistant 23 kb DNA hybridized insitu specifically with the heterochromatic chromocentre. It appears that the digestibility of heterochromatin region in genome ofDrosophila nasuta with the tested restriction enzymes is dependent on the availability of their recognition sites.

    • Author Affiliations

       

      P K Tiwari1 2 S C Lakhotia1

      1. Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi - 221 005, India
      2. School of Studies in Zoology, Jiwaji University, Gwalior - 474 011, India
    • Dates

       
  • Journal of Biosciences | News

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