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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/016/03/0161-0174

    • Keywords

       

      Bacteriophage M B78; Salmonella phage; restriction map; DNA packaging; ‘pac’ site

    • Abstract

       

      Bacteriophage MB78 is a virulent phage ofSalmonella typhimurium. The viral DNA is 42 kb in size and seems to be circularly permuted. We show that viral DNA replication is through concatemeric DNA formation which is subsequently converted into full length DNA through headful packaging. A restriction map of MB78 DNA for six restriction endonucleases e.g.BgIII,PvuII, ECORI, ClaI, SalI and SmaI has been constructed. The yield of certain fragments in less than molar amount is explained in terms of permutation and the headful mechanism of packaging. The packaging site (pac site) has been suggested.

    • Author Affiliations

       

      Saeed A Khan1 2 S S Murty1 Manzoor A Zargar1 M Chakravorty1

      1. Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi - 221 005, India
      2. C/o Philip Serwar, Department of Biochemistry, University of Texas, Health Sciences Centre at San Antonio, Texas - 78284, USA
    • Dates

       
  • Journal of Biosciences | News

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