• Two forms of trehalase in rabbit enterocyte: Purification and chemical modification

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    • Keywords

       

      Trehalase; brush border membrane; basolateral membrane; chemical modification; rabbit

    • Abstract

       

      Trehalase found to be associated with the brush border membrane vesicles and the Ca2+ aggregated basolateral membrane vesicles were purified to homogeneity. They were found to differ in their molecular weight, subunit structure, heal stability, N-terminal residues, amino acid composition and also the active site residues. Chemical modification showed the presence of a histidine and tyrosine at the active site of brush border membrane vesicle trehalase and two histidines at the active site of basolateral membrane vesicle.

    • Author Affiliations

       

      S Sanker1 2 S Sivakami1

      1. Department of Life Sciences, University of Bombay, Vidyanagari, Santacruz (E), Bombay - 400 098, India
      2. Molecular Biophysics Unit, Indian Institute of Science, Bangalore - 560 012, India
    • Dates

       
  • Journal of Biosciences | News

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