• Organization and chromosomal localization ofβ-tubulin genes inLeishmania donovani

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    • Keywords

       

      Leishmania donovani ; β-tubulin; gene organization; chromosome location; DNA sequence

    • Abstract

       

      The genomic organization and chromosomal location of theβ-tubulin isogenes inLeishmania donovani promastigotes has been studied by nucleic acid hybridization techniques using a cloned β-tubulin gene. We have cloned aβ-tubulin gene fragment, 3.3 kbp long, from genomic DNA ofLeishmania donovani using a heterologousβ-tubulin DNA as probe. Restriction maps of this clone have been prepared. It has been estimated that there are approximately 11–15 copies of theβ-tubulin genes per haploid genome. The majority of these isogenes are arranged in a tandem repeat with a length of 3.5 kbp on a single chromosome. In addition a few dispersed gene copies at different chromosomal loci were detected by pulse field gradient gel electrophoresis. Part of the internal coding region of the gene has been sequenced to confirm the identity of theβ-tubulin clone and is found to be nearly identical to that ofLeishmania mexicana amazonensis.

    • Author Affiliations

       

      Saumitra Das1 Samit Adhya1

      1. Leishmania Group, Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Calcutta - 700 032, India
    • Dates

       
  • Journal of Biosciences | News

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