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    • Dephosphorylation of cell-surface phosphoproteins of goat spermatozoa

      M Barua G C Majumder

      Volume 15 Issue 3 September 1990 pp 217-221

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/015/03/0217-0221

    • Keywords

       

      Ecto-protein; spermatozoa; phosphoproteins; protein dephosphorylation; Proteinphosphatase; cell-surface

    • Abstract

       

      Multiple ecto-phosphoproteins of the goat cauda-epididymal intact spermatozoa have been shown to undergo dephosphorylationin vitro by endogenous phosphoprotein phosphatase(s) located on the sperm outer surface. The major ecto-phosphoproteins that are dephosphorylated have molecular masses of 27, 40, 70, 116 and 205 kDa. The cell surface dephosphorylation reaction is not dependent on bivalent metal ions. Mg2+ (5 mM), Mn2+ (5 mM), orthovanadate (200ΜM) and cAMP (5 ΜM) have no effect on this surface reaction whereas it is inhibited nearly 50% by Co2+ or Zn2+ (1 mM). Spermidine (5 mM), or Ca2+ (1mM) inhibited to a small extent (approx. 25%) the cell surface dephosphorylation of proteins.

    • Author Affiliations

       

      M Barua1 G C Majumder1

      1. Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Jadavpur, Calcutta - 700 032, India

    • Dates

       
      Published
      September 1990

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