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    • Calcium and magnesium induced changes in the relative fluidity of phosphatidylcholine liposomes

      R K Mishra Gauri S Singhal

      Volume 15 Issue 3 September 1990 pp 193-197

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/015/03/0193-0197

    • Keywords

       

      Liposomes; fluidity; fluorescence polarization; phosphatidylcholine

    • Abstract

       

      The effect of Ca2+ and Mg2+ on relative fluidity of phosphatidylcholine liposomes was studied by measuring the degree of chlorophyll fluorescence polarization. An increase in the degree of fluorescence polarization was observed on incubation of liposomes with different concentrations of Ca2+ or Mg2+. The results have been interpreted on the basis of increase in the size of liposomes which could be brought about by calcium or magnesium induced fusion of small unilamellar liposomes to form larger vesicles. Fusion of liposomes has also been confirmed by the experiments on efficiency of energy transfer from chlorophyll b to chlorophyll a, and transmission electron microscopy of liposomes before and after incubation with Ca2+ and Mg2+.

    • Author Affiliations

       

      R K Mishra1 Gauri S Singhal1

      1. School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110 067, India

    • Dates

       
      Published
      September 1990

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