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    • Fluidity of detergent micelles plays an important role in muscarinic receptor solubilization

      Anu KŎiv Ago Rinken Jaak Järv

      Volume 15 Issue 3 September 1990 pp 149-152

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/015/03/0149-0152

    • Keywords

       

      Muscarinic receptor; receptor solubilization; diphenylhexatriene; fluoresence; polarization; micelle fluidity

    • Abstract

       

      In order to find a suitable reagent for extracting the muscarinic receptor from rat brain membranes 14 different detergents were tested. Only the plant glycoside digitonin efficiently solubilized the receptor protein in its native form. At the same time microviscosity of detergent micelles was determined by measuring the fluorescence polarization of a hydrophobic fluorescent probe diphenylhexatriene incorporated into the micelles. In the case of digitonin the polarization value was close to the corresponding value obtained for rat brain membrane fragments, while for the other detergents studied it remained considerably lower. The results obtained indicate that the fluidity of detergent micelles may play an important role in retaining the active conformation of the solubilized muscarinic receptor.

    • Author Affiliations

       

      Anu KŎiv1 Ago Rinken1 Jaak Järv1

      1. Laboratory of Bioorganic Chemistry, Tartu University, Tartu, Estonia, USSR

    • Dates

       
      Published
      September 1990

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