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    • Keywords


      Sperms; ESR spin labelling; [31P] NMR; human semen; goat reproductive organ; electron transport chain; cold shock

    • Abstract


      Motility is used as a routine parameter for assessing spermatozoa activity. The quality rating techniques adopted are based on electron or optical microscopy. However, these methods depend on gross structural and dynamical features of sperm cells and do not provide information on metabolic activity of intact cells. Lately, biochemical assays have become popular. Such methods are cumbersome and destroy the samples. Magnetic resonance methods offer a non-invasive method for studies on intact sperms. We have investigated respiration, maturation andin vitro capacitation of sperms from human ejaculates and sperms extracted from goat reproductive organ using electron spin resonance spin labelling and [31P] nuclear magnetic resonance methods. These studies clearly establish the advantages of magnetic resonance in studies related to metabolic activity of sperms.

    • Author Affiliations


      Ratna S Phadke1 Sudha Srivastava1 Girjesh Govil1

      1. Chemical Physics Group, Tata Institute of Fundamental Research, Bombay - 400 005, India
    • Dates

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