• Characterization of cytosolic phosphoglucoisomerase from immature wheat (Triticum aestivum L.) endosperm

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      https://www.ias.ac.in/article/fulltext/jbsc/014/01/0047-0054

    • Keywords

       

      Wheat; Triticum aestivum ; endosperm; cytosol; phosphoglucoisomerase

    • Abstract

       

      Phosphoglucoisomerase from cytosol of immature wheat endosperm was purified 650-fold by ammonium sulphate fractionation, isopropyl alcohol precipitation, DEAE-cellulose chromatography and gel filtration through Sepharose CL-6B. The enzyme, with a molecular weight of about 130,000, exhibited maximum activity at pH 8.1. It showed typical hyperbolic kinetics with both fructose 6-P and glucose 6-P withKm of 0.18 mM and 0.44mM respectively. On either side of the optimum pH, the enzyme had lower affinity for the substrates. Using glucose 6-P as the substrate, the equilibrium was reached at 27% fructose 6-P and 73% glucose 6-P with an equilibrium constant of 2.7. The ΔF calculated from the apparent equilibrium constant was +597 cal mol-1. The activation energy calculated from the Arrhenius plot was 5500 cal mol-1. The enzyme was completely inhibited by ribose 5-P, ribulose 5-P and 6-phosphogluconate, withKi values of 0.17, 0.25 and 0.14 mM respectively. The probable role of the enzyme in starch biosynthesis is discussed.

    • Author Affiliations

       

      R S Sangwan1 Randhir Singh1

      1. Department of Chemistry and Biochemistry, Haryana Agricultural University, Hisar - 125 004, India
    • Dates

       
  • Journal of Biosciences | News

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