• Correlation of bacterial viability with uptake of [14C] acetate into phenolic glycolipid-1 ofMycobacterium leprae within Schwannoma cells

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    • Keywords


      Mycobacterium leprae ; Schwannoma cell line; lipid metabolism

    • Abstract


      The viability ofMycobacterium leprae, maintained within 33B Schwannoma cells, was estimated in terms of incorporation of [14C] acetate into its specific phenolic glycolipid-1. This measure of viability was correlated with two other assays,viz., fluorescein diacetate/ethidium bromide staining and mouse footpad growth. Observation of a 2-fold increase in the number of intracellularMycobacterium leprae over an experimental period of 12 days also corroborated this contention. Furthermore, on addition of anti-leprosy drugs to these intracellularMycobacterium leprae there was significant decrease in phenolic glycolipid-1 synthesis indicative of loss of viability of the organisms. This study also established the importance of the host cell for active bacillary metabolism, asMycobacterium leprae maintained in cell-free conditions showed no incorporation into phenolic glycolipid-1. Moreover, compromising the host’s protein synthesis capacity with cycloheximide, also led to reduction in bacillary metabolism. As this system measures the metabolic synthesis of a uniqueMycobacterium leprae component, it would be useful for development and screening of compounds acting against specific bacillary targets.

    • Author Affiliations


      Y Mistry1 N H Antia1 R Mukherjee1 2

      1. The Foundation for Medical Research, 84-A, R. G. Thadani Marg, Worli, Bombay - 400 018, India
      2. Department of Microbiology, National Institute of Immunology, J. N. U. Complex, Shahid Jeet Singh Marg, New Delhi - 110 067, India
    • Dates

  • Journal of Biosciences | News

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