• Stick enzyme-linked immunosorbent assay using the avidin-biotin system for detection of circulating antigen in bancroftian filariasis

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      https://www.ias.ac.in/article/fulltext/jbsc/013/03/0229-0233

    • Keywords

       

      Wuchereria bancrofti ; filarial serum immunoglobulin G; enzyme-linked immunosorbent assay; biotinylated FSIgG; biotinylated ES antigen; avidin penicillinase

    • Abstract

       

      Detection of filarial antigen in different groups of sera was carried out by sandwich as well as inhibition enzyme-linked immunosorbent assays using antibody-coated sticks. Both systems were found to be equally sensitive in detecting antigen in 90% of microfilariae carriers. Incorporation of avidin-biotin in the sandwich assay system increased the sensitivity of antigen detection from 10−6 to 10−16 pg. A 67% decrease in the number of false negative results was observed when the sensitive avidin-biotin inhibition enzyme-linked immunosorbent assay system was used for analysis of filaria blood samples.

    • Author Affiliations

       

      K A Parkhe1 P Ramaprasad1 B C Harinath1

      1. Mahatma Gandhi Institute of Medical Sciences, Sevagram - 442 102, India
    • Dates

       
  • Journal of Biosciences | News

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