• Isolation, purification and partial characterisation of prealbumin from cerebrospinal fluid

    • Fulltext


        Click here to view fulltext PDF

      Permanent link:

    • Keywords


      Prealbumin; protein purification; gel electrophoresis; gel filtration; Immunoelectrophoresis; amino acid analysis

    • Abstract


      Prealbumin from human cerebrospinal fluid was purified using a combination of ammonium sulphate precipitation, phenol precipitation, Polyacrylamide disc gel electrophoresis and gel filtration on Sephadex G-100. The homogeneity of the purified protein was established by Polyacrylamide gel electrophoresis and Immunoelectrophoresis. On the basis of its molecular weight (55,000), amino acid composition, electrophoretic mobility and immunological cross-reactivity, the prealbumin from cerebrospinal fluid showed complete identity with serum prealbumin. The cerebrospinal fluid prealbumin levels in various neurological disorders may have a diagnostic significance.

    • Author Affiliations


      M V Bimanpalli1 P S Ghaswala1 2

      1. Department of Biochemistry, Grant Medical College, Bombay - 400 008, India
      2. Sir Hurkisondas Nurrotumdas Medical Research Society, Raja Rammohan Roy Road, Bombay - 400 004, India
    • Dates

  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.