• Purification and properties of trehalase from monkey small intestine

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/013/02/0153-0158

    • Keywords

       

      Trehalase; intestinal brush border membrane; hydrophobic protein

    • Abstract

       

      Brush border membrane trehalase was purified from monkey small intestine by a procedure which includes solubilisation by Triton X-100, ammonium sulphate fractionation, and chromatography on DE-52 and hydroxyapatite. The purified enzyme had a specific activity of 11 units/mg protein and was purified 140-fold. The enzyme showed a single protein band on Polyacrylamide gel electrophoresis. It had aKm value of 17.4 mM for trehalose and a Vmax of 1.33 units. Sucrose and Tris acted as competitive inhibitors of the enzyme.

    • Author Affiliations

       

      S Sanker1 S Sivakami1

      1. Department of Life Sciences, University of Bombay, Vidyanagari, Santacruz (East), Bombay - 400 098, India
    • Dates

       
  • Journal of Biosciences | News

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