• Cloning and characterization of a DNA repair gene inHaemophilus influenzae

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      https://www.ias.ac.in/article/fulltext/jbsc/012/02/0115-0123

    • Keywords

       

      Haemophilus influenzae ; self-cloning; uvr1 gene; directed mutagenesis

    • Abstract

       

      Using a high-efficiency DNA cloning vector pJ1–8, a DNA repair geneuvr1 has been self-cloned in bacteriumHaemophilus influenzae. Chimeric plasmid pKuvrl, carrying wild type allele ofuvr1 gene and flanking DNA sequences, specifically complements auvr1 gene mutation in the bacterial chromosome. Auvr1} mutation could be transferred from chromosome byin vivo recombination to pKuvr1 and isolated and designated as plasmid pKuvrl. Plasmid pKuvrl carries a 11.3 kb chromosomal DNA insert which was scanned for the presence of any other DNA repair genes by a novel method of directed mutagenesis. Preliminary analysis of the 3 new mutants isolated by this method supports the notion that the insert contains more than one gene concerned with ultraviolet radiation-sensitivity.

    • Author Affiliations

       

      Rohinee P Kanade1 N K Notani1

      1. Molecular Biology and Agriculture Division, Bhabha Atomic Research Centre, Bombay - 400 085, India
    • Dates

       
  • Journal of Biosciences | News

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