• Importance of determining viability ofMycobacterium leprae inside macrophages—anin vitro method using uracil

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/011/01-04/0455-0463

    • Keywords

       

      Uracil uptake; Mycobacterium lePrae ; macroPhages; viability assay

    • Abstract

       

      It has been demonstrated thatMycobacterium leprae, are caPable of taking uP uracil and incorPorating it into trichloroacetic acid-insoluble materials, both as free susPension of bacteria, as well as when they are inside the macrophages, a host cell for theirin vivo survival. Same amount of bacteria show better incorPoration inside macroPhages than as free bacterial susPension. Both tyPes of incorPoration are inhibited by rifamPicin an antileProsy drug and an RNA synthesis inhibitor. Thus uracil uPtake byMycobacterium lePrae inside macroPhages has been used for standardising a raPidin vitro viability assay for the leProsy causing bacteria.

    • Author Affiliations

       

      Sunanda Vejare1 P R Mahadevan1

      1. The Foundation for Medical Research, 84-A, R. G. Thadani Marg, Worli, Bombay - 400 018, India
    • Dates

       
  • Journal of Biosciences | News

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