• Interaction of Cibacron Blue F3G-A and Procion Red HE-3B with sheep liver 5,10-methylenetetrahydrofolate reductase

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    • Keywords


      Cibacron Blue F3G-A; Procion Red HE-3B; 5,10-methylenetetrahydrofolate reductase

    • Abstract


      Cibacron Blue F3G-A, a probe used to monitor nucleotide binding domains in enzymes, inhibited sheep liver 5,10-methylenetetrahydrofolate reductase competitively with respect to 5-methyltetrahydrofolate and NADPH. TheKi values obtained by kinetic methods and theKd value for the binding of the dye to the enzyme estimated by protein fluorescence quenching were in the range 0.9–1.2 μM. Another triazine dye, Procion Red HE-3B interacted with the enzyme in an essentially similar manner to that observed with Cibacron Blue F3G-A. These results as well as the interaction of the dye with the enzyme monitored by difference spectroscopy and intrinsic protein fluorescence quenching methods indicated that the dye was probably interacting at the active site of the enzyme by binding at a hydrophobic region.

    • Author Affiliations


      K Varalakshmi1 H S Savithri1 N Appaji Rao1

      1. Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012, India
    • Dates

  • Journal of Biosciences | News

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