• The positional specificities of the oxygenation of linoleic acid catalyzed by two forms of lipoxygenase isolated from Bengal gram (Cicer arietinum)

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    • Keywords


      Lipoxygenase; Bengal gram

    • Abstract


      The products generated from linoleic acid by the two forms of Bengal gram lipoxygenase, BGL1 and BGL2, were analysed by high-performance liquid chromatography using μ-porasil column with isooctane containing 0.5% ethanol as the solvent system. The l3-hydroperoxyoctadecadienoic acid and its 9-isomer which are known to be produced by soybean lipoxygenase-1 and the potato enzyme respectively were used as standards. The results show that BGL1 generated almost exclusively the l3-hydroperoxyoctadecadienoic acid while BGL2 produced both l3-and the 9-isomer in the ratio 21:79. The secondary keto derivatives formed in the BGL2 reaction were also separated by this technique.

    • Author Affiliations


      Alip Borthakur1 B Ganesh Bhat1 Candadai S Ramadoss1

      1. Biochemistry Section, Food Chemistry Department, Central Food Technological Research Institute, Mysore - 570 013, India
    • Dates

  • Journal of Biosciences | News

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