• Structural organization of cholera toxin gene and its expression in an environmental non-pathogenic strain ofVibrio cholerae

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    • Keywords


      Vibrio cholerae ; avirulent strain; toxin gene

    • Abstract


      Non-pathogenic, environmental strain ofVibrio cholerae, ELTOR Ogawa EW6 carries a copy of the cholera toxin gene in its chromosome. Restriction enzyme digestion followed by Southern blot analysis revealed that the structure of the cholera toxin gene in this organism is different from that found in the virulent strains. The xbaI site which has been found to be conserved in the cholera toxin of the virulent strains examined so far, is absent here. Results of the RNA dot blot analysis indicated that the cholera toxin gene in EW6 is transcribed much less efficiently compared to the cholera toxin gene present in the virulent strainVibrio cholerae classical Inaba 569B.

    • Author Affiliations


      Uma Bhattacharyya1 2 Amit Ghosh1 Ranajit K Ghosh1

      1. Indian Institute of Chemical Biology, Jadavpur, Calcutta - 700 032, India
      2. Department of Biochemistry, University of North Carolina, Chapel Hill, North Carolina - 27514, USA
    • Dates

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