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      https://www.ias.ac.in/article/fulltext/jbsc/011/01-04/0155-0166

    • Keywords

       

      RNA processing; RNase III; RNA nucleotidyl transferase; T3 promoter

    • Abstract

       

      The primary transcripts synthesizedin vitro from a T3 DNA template byEscherichia coli RNA polymerase and by T3 phage-specific RNA polymerase have been characterized with regard to cleavage by RNase III and the size of the products of the cleavage reaction have been compared with those ofin vivo T3 RNAs. It has been observed that the large RNA molecule synthesized invitro byEscherichia coli RNA polymerase from the early region of T3 DNA are cleaved at specific sites byEscherichia coli RNase III to produce all the early mRNAs normally observed in T3-infected cells. In contrast, evidence presented here shows that some of the late T3 mRNAs are generated as direct products of transcription of late regions of T3 DNA by T3 RNA polymerase without mediation of RNase III, while many other late T3 mRNAs are formed by RNase III cleavage of two of the high molecular weight T3 RNA polymerase transcripts. Thesein vitro data appear to be in good agreement with the observed sizes of late T3 mRNAs formedin vivo in T3-infected RNase III-deficient and RNaseIII+Escherichia coli cells.

    • Author Affiliations

       

      Hemanta K Majumder1 2 Samit ADhya1 2 Umadas Maitra1

      1. Department of Developmental Biology and Cancer, Division of Biology, Albert Einstein College of Medicine, Bronx, New York - 10461, USA
      2. Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Calcutta - 700 032, India
    • Dates

       
  • Journal of Biosciences | News

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