• Effect ofcis andtrans unsaturated fatty acids on the transport properties ofSalmonella typhimurium

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      https://www.ias.ac.in/article/fulltext/jbsc/011/01-04/0145-0153

    • Keywords

       

      Transport; fatty acid auxotroph; cis andtrans-unsaturated fatty acid

    • Abstract

       

      The transport of α-methyl-D-glucoside and two aminoacids, L-phenylalanine and L-leucine by a temperature sensitive fatty acid requiring mutant ofSalmonella typhimurium was studied under conditions of supplementation withcis or trans-unsaturated fatty acids. The results of such experiments definitely establish a relationship between the fatty acids composition of the membrane and the transport property of the cells. Cells grown in the presence of trans-unsaturated fatty acids cannot transport so efficiently as compared to the cis-unsaturated fatty acid-grown cells except linolelaidic acid, atrans-trans-unsaturated fatty acid. Protein: phospholipid ratio of the membrane also varies significantly under such conditions. The affinity of L-phenylalanine transport carrier for the substrate changes remarkably in cells grown in the presence of differentcis or trans-unsaturated fatty acids and indicate the possible role of membrane lipids in membrane assembly as well as regulation of the activity of L-phenylalanine transport system.

    • Author Affiliations

       

      J K Deb1 2 R Goyal1 M Chakravorty1

      1. Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi - 221 005, India
      2. Department of Chemistry, Indian Institute of Technology, New Delhi - 110029, India
    • Dates

       
  • Journal of Biosciences | News

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