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Wuchereria bancrofti ; bancroftian filariasis; excretory-secretory antigens; monoclonal antibodies; enzyme linked immunosorbent assay; circulating filarial antigen

Two monoclonal antibodiesWuchereria bancrofti E 33 andWuchereria bancrofli E 34 raised againstWuchereria bancrofti microfilarial excretory-secretory antigens were studied for their diagnostic utility.Wuchereria bancrofti E 34 monoclonal antibody was found to be relatively specific and sensitive in detection of circulating filarial antigen. WhenWuchereria bancrofti E 34 monoclonal antibody was used alongwith immunoglobulin G fraction of human filarial serum immunoglobulins in double antibody sandwich enzyme linked immunosorbent assay. 68% of microfilaraemic sera (26 out of 38). 12% of clinical filarial sera (3 out of 25), 13% endemic normal sera (2 out of 15) and none of the 20 non-endemic normal sera showed the presence of filarial antigen. The filarial antigen detected byWuchereria bancrofti E 34 monoclonal antibody in double antibody sandwich enzyme linked immunosorbent assay is possibly associated with the active stage (microfilaraemia) of infection.

M V R Reddy¹ P Rama Prasad¹ W F Piessens^{1 2} B C Harinath¹

1. Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Maharashtra - 442 102, India
2. Department of Tropical Public Health, Harvard School of Public Health, 665, Huntington Avenue, Boston, Massachusetts - 02115, USA

Published

December 1986

Manuscript received

16 December 1985

Manuscript revised

12 August 1986