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      https://www.ias.ac.in/article/fulltext/jbsc/010/04/0461-0466

    • Keywords

       

      Wuchereria bancrofti ; bancroftian filariasis; excretory-secretory antigens; monoclonal antibodies; enzyme linked immunosorbent assay; circulating filarial antigen

    • Abstract

       

      Two monoclonal antibodiesWuchereria bancrofti E 33 andWuchereria bancrofli E 34 raised againstWuchereria bancrofti microfilarial excretory-secretory antigens were studied for their diagnostic utility.Wuchereria bancrofti E 34 monoclonal antibody was found to be relatively specific and sensitive in detection of circulating filarial antigen. WhenWuchereria bancrofti E 34 monoclonal antibody was used alongwith immunoglobulin G fraction of human filarial serum immunoglobulins in double antibody sandwich enzyme linked immunosorbent assay. 68% of microfilaraemic sera (26 out of 38). 12% of clinical filarial sera (3 out of 25), 13% endemic normal sera (2 out of 15) and none of the 20 non-endemic normal sera showed the presence of filarial antigen. The filarial antigen detected byWuchereria bancrofti E 34 monoclonal antibody in double antibody sandwich enzyme linked immunosorbent assay is possibly associated with the active stage (microfilaraemia) of infection.

    • Author Affiliations

       

      M V R Reddy1 P Rama Prasad1 W F Piessens1 2 B C Harinath1

      1. Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Maharashtra - 442 102, India
      2. Department of Tropical Public Health, Harvard School of Public Health, 665, Huntington Avenue, Boston, Massachusetts - 02115, USA
    • Dates

       
  • Journal of Biosciences | News

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