• A method for the isolation of intact Sertoli cell-germ cell associations from rat seminiferous tubules and their further partition into Sertol cell and germ cell fractions

    • Fulltext


        Click here to view fulltext PDF

      Permanent link:

    • Keywords


      Rat seminiferous tubules; Sertoli cells; germ cells; isolation

    • Abstract


      A technique is described for obtaining a Sertoli cell-enriched and a germ cell-enriched fraction from immature rat testes. Sertoli cell-germ cell associations were obtained by incubating washed seminiferous tubule fragments with Collagenase and Pancreatin. They were then manually dissociated into a suspension comprising Sertoli cells as well as the various germ cell types characteristic for a given day of ontogeny. Fractionation into a Sertoli cell-enriched fraction and a germ cell-enriched fraction was effected by centrifugation following layering over a stepwise gradient of Ficoll-400. While the time-span compares favourably with other procedures reported in the literature, it is believed this is the first time a method is described that enables the simultaneous recovery of both the Sertoli cells and the germ cells.

    • Author Affiliations


      MeenaKumari1 S Duraiswami1

      1. Department of Zoology, University of Delhi, Delhi - 10 007, India
    • Dates

  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.