• A method for the isolation of intact Sertoli cell-germ cell associations from rat seminiferous tubules and their further partition into Sertol cell and germ cell fractions

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      https://www.ias.ac.in/article/fulltext/jbsc/010/03/0413-0422

    • Keywords

       

      Rat seminiferous tubules; Sertoli cells; germ cells; isolation

    • Abstract

       

      A technique is described for obtaining a Sertoli cell-enriched and a germ cell-enriched fraction from immature rat testes. Sertoli cell-germ cell associations were obtained by incubating washed seminiferous tubule fragments with Collagenase and Pancreatin. They were then manually dissociated into a suspension comprising Sertoli cells as well as the various germ cell types characteristic for a given day of ontogeny. Fractionation into a Sertoli cell-enriched fraction and a germ cell-enriched fraction was effected by centrifugation following layering over a stepwise gradient of Ficoll-400. While the time-span compares favourably with other procedures reported in the literature, it is believed this is the first time a method is described that enables the simultaneous recovery of both the Sertoli cells and the germ cells.

    • Author Affiliations

       

      MeenaKumari1 S Duraiswami1

      1. Department of Zoology, University of Delhi, Delhi - 10 007, India
    • Dates

       
  • Journal of Biosciences | News

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