1. Home
    2. Journals
    3. Journal of Biosciences
    4. Volume 10
    5. Issue 3

    • Use of epoxysepharose for protein immobilisation

      G S Murthy N R Moudgal

      Volume 10 Issue 3 September 1986 pp 351-358

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/010/03/0351-0358

    • Keywords

       

      Epoxy Sepharose; affinity matrix; protein immobilisation

    • Abstract

       

      Epoxy Sepharose, an activated affinity matrix which has been used for immobilisation of carbohydrates has been tried for immobilisation of proteins. Under normal conditions of coupling at neutral or alkaline pH proteins do not couple to epoxy Sepharose. However, a very high salt concentration during coupling allows the binding of proteins to epoxy Sepharose at a pH as low as 8.5. Increasing ionic strength and/or pH facilitates the binding. The bioactivity of the proteins is not destroyed by the immobilisation. This matrix, unlike cyanogen bromide-Sepharose, retains its ability to bind albumin by 80–90% even after 60 days of storage in aqueous suspension at 4°C. Its capacity to bind proteins is comparable to that of cyanogen bromide-Sepharose.

    • Author Affiliations

       

      G S Murthy1 N R Moudgal1

      1. Center for Advanced Research in Reproductive Biology (ICMR) Laboratory of Endocrine Biochemistry Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012, India

    • Dates

       
      Published
      September 1986
      Manuscript received
      3 August 1985
      Manuscript revised
      15 May 1986

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
      <

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2022-2023 Indian Academy of Sciences, Bengaluru.

Contact | Site index