• Use of epoxysepharose for protein immobilisation

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      https://www.ias.ac.in/article/fulltext/jbsc/010/03/0351-0358

    • Keywords

       

      Epoxy Sepharose; affinity matrix; protein immobilisation

    • Abstract

       

      Epoxy Sepharose, an activated affinity matrix which has been used for immobilisation of carbohydrates has been tried for immobilisation of proteins. Under normal conditions of coupling at neutral or alkaline pH proteins do not couple to epoxy Sepharose. However, a very high salt concentration during coupling allows the binding of proteins to epoxy Sepharose at a pH as low as 8.5. Increasing ionic strength and/or pH facilitates the binding. The bioactivity of the proteins is not destroyed by the immobilisation. This matrix, unlike cyanogen bromide-Sepharose, retains its ability to bind albumin by 80–90% even after 60 days of storage in aqueous suspension at 4°C. Its capacity to bind proteins is comparable to that of cyanogen bromide-Sepharose.

    • Author Affiliations

       

      G S Murthy1 N R Moudgal1

      1. Center for Advanced Research in Reproductive Biology (ICMR) Laboratory of Endocrine Biochemistry Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012, India
    • Dates

       
  • Journal of Biosciences | News

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