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    • Cross reactivity and enzyme sensitivity of immunoaffinity purified Sm/RNP antigens

      Ashok Kumar Rashid Ali

      Volume 10 Issue 2 June 1986 pp 235-242

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/010/02/0235-0242

    • Keywords

       

      Sm/RNP; hnRNP; autoantibodies; enzyme-linked immunosorbent assay; enzyme sensitivity

    • Abstract

       

      Immunoaffinity purified Sm/RNP antigens from buffalo and goat liver were studied to determine the role of RNA and proteins towards the antigenicity of Sm and RNP antigens. A more direct approach using enzyme-linked immunosorbent assay on nylon beads has been utilized to look into the problem. The effect of enzyme treatment and the role of RNA and protein fractions in influencing antigenicity have been described. RNA seems to be involved in the maintenance of RNP specific polypeptides in suitable conformation so as to keep them in solution. Removal of RNA leads to insolubilization of RNP specific polypeptides. Antibodies to Sm and RNP antigens have been shown to cross react with poly A containing heterogeneous nuclear ribonucleoprotein with no cross reactivity with thymus RNA or DNA.

    • Author Affiliations

       

      Ashok Kumar1 2 Rashid Ali1

      1. Department of Biochemistry, J.N. Medical College, Aligarh Muslim University, Aligarh - 202001, India
      2. Department of Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna - 800014, India

    • Dates

       
      Published
      June 1986
      Manuscript received
      29 April 1985
      Manuscript revised
      28 January 1986

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