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      https://www.ias.ac.in/article/fulltext/jbsc/010/02/0215-0225

    • Keywords

       

      Lysosomal enzyme binding protein; brain; immobilisation; binding requirements

    • Abstract

       

      The lysosomal enzyme binding protein (receptor protein) isolated from monkey brain was immobilised on Sepharose 4B and used to study the binding of brain lysosomal enzymes. The immobilised protein could bind \-D-glucosaminidase, α-D-mannosidase, α-L-fucosidase and²-D-glucuronidase. The bound enzymes could be eluted either at an acid pH of 4.5 or by mannose 6-phosphate but not by a number of other sugars tested. Binding could be abolished by prior treatment of the lysosomal enzymes with sodium periodate. Alkaline phosphatase treatment of the enzymes did not prevent the binding of the lysosomal enzymes to the column but decreased their affinity, as seen by a shift in their elution profile, when a gradient elution with mannose 6-phosphate was employed. These results suggested that an ‘uncovered’ phosphate on the carbohydrate moiety of the enzymes was not essential for binding but can enhance the binding affinity.

    • Author Affiliations

       

      Keith Alvares1 K Panneerselvam1 A S Balasubramanian1

      1. Neurochemistry Laboratory, Department of Neurological Sciences, Christian Medical College Hospital, Vellore - 632004, India
    • Dates

       
  • Journal of Biosciences | News

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