• Correlation between riboflavin carrier protein induction and its mRNA activity in estrogen stimulated chicken liver and oviduct

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    • Keywords

       

      Primary translation product; cell-free translation; rabbit reticulocyte lysate; stripped microsomal membrane; progesterone; estradiol-17β; precursor; poly A+ -RNA

    • Abstract

       

      Poly A enriched RNA from either liver or oviduct of estradiol-17β treated immature chicks supported [3H]-leucine incorporation into immunoprecipitable riboflavin carrier protein in a dose-dependent manner when translated in the rabbit reticulocyte lysate system. Primary translation product of riboflavin carrier protein had a molecular weight of 38,000 which on incubation with a stripped hepatic microsomal preparation was processed to a product with a size comparable to native riboflavin carrier protein. Poly A enriched RNA from both the liver and the oviduct of estrogen-treated birds stimulated [3H]-leucine incorporation into riboflavin carrier protein and this was 2–3 fold higher during secondary stimulationvis-a-vis primary stimulation with the steroid. Poly A enriched RNA from the liver of progesteronetreated birds during secondary stimulation did not support riboflavin carrier protein synthesis. In contrast, poly A enriched RNA from the oviduct of the birds treated with progesterone during secondary (but not primary) stimulation did exhibit riboflavin carrier protein-mRNA activity which was comparable to that stimulated by estradiol-17β

    • Author Affiliations

       

      B Durga Kumari1 2 P R Adiga1

      1. Department of Biochemistry, Indian Institute of Science, Bangalore - 560012, India
      2. Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, California - 92037, USA
    • Dates

       
  • Journal of Biosciences | News

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