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    • α-galactosidase from germinating guar (Cyamopsis tetragonolobus) seeds

      B D Shivanna M Ramakrishna

      Volume 9 Issue 1-2 September 1985 pp 109-116

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/009/01-02/0109-0116

    • Keywords

       

      Guar; Cyamopsis tetragonolobus ; Leguminoseae, germination; α-galactosidase

    • Abstract

       

      The changes in α-galactosidase activity in guar (Cyamopsis tetragonolobus) seeds was followed during seven days of germination. The enzyme activity was maximal on the first day of germination and gradually decreased during subsequent days. On the second day of germination the partially purified enzyme upon ion-exchange chromatography on CM-Sephadex C-50 was resolved into α-galactosidase-A (anionic), α-galactosidase-C1 (cationic) and α-galactosidase-C2 (cationic) and their relative proportions were 28,12 and 60%, respectively. The combined α-galactosidase C1 and C2 activities increased in the first two days of germination followed by significant decrease after the 3rd day onwards, whereas α-galactosidase-A remained fairly constant throughout the germination period, α-Galactosidase-A and C2 had differentKm and Vmax values withp-nitrophenyl α-D-galactopyranoside, raffinose and melibiose as substrates and also differed in their thermal stabilities

    • Author Affiliations

       

      B D Shivanna1 M Ramakrishna1

      1. Department of Food Chemistry, Biochemistry Section, Central Food Technological Research Institute, Mysore - 570013, India

    • Dates

       
      Published
      September 1985
      Manuscript received
      29 June 1985

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