• Activity of radiation degradation products of vitamins A and E to haemolyse erythrocyte

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      https://www.ias.ac.in/article/fulltext/jbsc/007/03-04/0303-0313

    • Keywords

       

      Vitamin A; vitamin E; radiation degradation products; erythrocyte haemolysis

    • Abstract

       

      Exposure of vitamin A acetate in freely dissolved state to γ-radiationin vitro caused a dose dependent degradation accompanied by the formation of new products. The radiation degradation products were separated by chromatography using step gradient elution. The parent molecule, vitamin A acetate, induced negligible haemolysis of erythrocytes. In contrast, the polar products formed by irradiation were found to be potent haemolysing agents. A highly polar product, eluted with methanol revealed maximum haemolytic activity. Acetylation of these products resulted in loss of their haemolytic properties. Similarly, vitamin E acetate, a known stabilizer of the biomembranes, after irradiation yielded products which caused haemolysis of erythrocytes. It was demonstrated that irradiation introduces hydroxyl groups which impart haemolytic properties to the radiation degradation products of vitamin A

    • Author Affiliations

       

      Brij Bhushan1 V Ninjoor G B Nadkarni1

      1. Biochemistry and Food Technology Division, Bhabha Atomic Research Centre, Trombay, Bombay - 400085, India
    • Dates

       
  • Journal of Biosciences | News

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