• A solid phase radioimmunoassay for detection of early pregnancy in the South Indian bonnet monkeyMacaca radiata

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      https://www.ias.ac.in/article/fulltext/jbsc/007/02/0145-0152

    • Keywords

       

      Chorionic gonadotropin; monkey; solid phase assay; ovine leutinizing hormone; antiserum

    • Abstract

       

      Using an antibody raised in the rabbit to ovine leutenizing hormone β subunit coupled to activated cellulose, a solid phase radioimmunoassay to detect early pregnancy in the South Indian bonnet monkey has been developed. Non-specific inhibition due to serum was eliminated by inclusion of new born calf serum in the assay tubes. The assay is simple, needs only one centrifugation and can be completed in 6 h at room temperature with no false positive results.

    • Author Affiliations

       

      R Chakrabarti1 G S Murthy1 A Jagannadha Rao1

      1. Department of Biochemistry, Center for Advanced Research in Reproductive Biology (ICMR), Indian Institute of Science, Bangalore - 560 012, India
    • Dates

       
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