Isolation, characterization and mapping of temperature-sensitive mutants of mycobacteriophage I3
Chanchal Sadhu Mohan lal Gope Krishna Sadhu K P Gopinathan
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Eighteen temperature-sensitive mutants of mycobacteriophage I3 have been isolated and partially characterized. All the mutants were defective in vegetative replication. Based on temperature shift experiments with the temperature sensitive mutants, the thermosensitive phase of the phage development period has been characterized for each mutant. The genes have been mapped by recombination analysis. The early, continuous and middle genes seem to cluster on the genetic map
Chanchal Sadhu1 Mohan lal Gope1 Krishna Sadhu1 K P Gopinathan1
Volume 48, 2023
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