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      https://www.ias.ac.in/article/fulltext/jbsc/006/06/0823-0828

    • Keywords

       

      Concanavalin-A; fluorescence; selective excitation; 305 nm.

    • Abstract

       

      Using the lectin-concanavalin-A, the tryptophan fluorescence as a function of pH was studied. The pH dependent, fluorescence intensity changes were significantly higher when excited at 305 nm, than when irradiated at 280nm. Only one tryptophanyl per monomer of concanavalin-A was available for oxidation by N-bromosuccinimide in the dimeric form at pH 4·9; no tryptophanyl could be oxidised in the demetallised dimer (pH 3·0) and native tetramer (pH 7·0). Based on this fluorescence data and the already known crystal structure data, it appears that tryptophanyl 88 in concanavalin-A may be selectively excited by 305 nm radiation

    • Author Affiliations

       

      M V R Rao1 M Atreyi1 M R Rajeswari1

      1. Department of Chemistry, University of Delhi, Delhi - 110 007, India
    • Dates

       
  • Journal of Biosciences | News

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