• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/006/06/0817-0821

    • Keywords

       

      DNA melting; formamide; T m ; DNA base composition; DNA reassociation

    • Abstract

       

      The utility of formamide in the denaturation and renaturation of DNA has been examined. The melting temperature of duplex DNA is lowered by 0·6°C per per cent formamide. The depression of melting temperature is independent of the GC content. Formamide also increases the width of the thermal transition. Upto 30%, it does not affect the rate of DNA reassociation

    • Author Affiliations

       

      Chanchal Sadhu1 Santanu Dutta1 2 K P Gopinathan1

      1. Microbiology and Cell Biology Laboratory, Indian Institute of Science, Bangalore - 560 012, India
      2. Molecular Biophysics Unit, Indian Institute of Science, Bangalore - 560 012, India
    • Dates

       
  • Journal of Biosciences | News

© 2017-2019 Indian Academy of Sciences, Bengaluru.