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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/006/06/0817-0821

    • Keywords

       

      DNA melting; formamide; T m ; DNA base composition; DNA reassociation

    • Abstract

       

      The utility of formamide in the denaturation and renaturation of DNA has been examined. The melting temperature of duplex DNA is lowered by 0·6°C per per cent formamide. The depression of melting temperature is independent of the GC content. Formamide also increases the width of the thermal transition. Upto 30%, it does not affect the rate of DNA reassociation

    • Author Affiliations

       

      Chanchal Sadhu1 Santanu Dutta1 2 K P Gopinathan1

      1. Microbiology and Cell Biology Laboratory, Indian Institute of Science, Bangalore - 560 012, India
      2. Molecular Biophysics Unit, Indian Institute of Science, Bangalore - 560 012, India

    • Dates

       
      Published
      December 1984
      Manuscript received
      5 May 1984
      Manuscript revised
      20 August 1984

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

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