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    • Keywords


      DNA melting; formamide; T m ; DNA base composition; DNA reassociation

    • Abstract


      The utility of formamide in the denaturation and renaturation of DNA has been examined. The melting temperature of duplex DNA is lowered by 0·6°C per per cent formamide. The depression of melting temperature is independent of the GC content. Formamide also increases the width of the thermal transition. Upto 30%, it does not affect the rate of DNA reassociation

    • Author Affiliations


      Chanchal Sadhu1 Santanu Dutta1 2 K P Gopinathan1

      1. Microbiology and Cell Biology Laboratory, Indian Institute of Science, Bangalore - 560 012, India
      2. Molecular Biophysics Unit, Indian Institute of Science, Bangalore - 560 012, India
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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