• Anin vitro test using cholesterol metabolism of macrophages to determine drug sensitivity and resistance ofMycobacterium leprae

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      https://www.ias.ac.in/article/fulltext/jbsc/006/02/0221-0231

    • Keywords

       

      Mycobacterium leprae; colesterol; cholesterol ester; macrophage; drug sensitivity/resistance

    • Abstract

       

      Macrophages that have ingested liveMycobacterium leprae show a preferential accumulation of cholesterol ester. Such an accumulation is not seen, on the ingestion of dead bacteria. Among the macrophages that ingest liveMycobacterium leprae, the presence of dapsone or rifampicin prevents largely the alteration in the anticipated increase in the cholesterol ester indicating the sensitivity of the bacteria to the drug. In the small number of relapsed patients, the presence of dapsone did not reduce the cholesterol ester increase, suggesting that theMycobacterium leprae present are either resistant or escaped detection. The method provides a rapid drug screening system foranti-Mycobacterium leprae activity of known and unknown compounds

    • Author Affiliations

       

      Ishwari Nair1 P R Mahadevan1

      1. The Foundation for Medical Research, 84-A, R. G. Thadani Marg, Worli, Bombay - 400 018, India
    • Dates

       
  • Journal of Biosciences | News

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