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    • Keywords


      Cell surface; DNA biosynthesis; DNA polymerase α; glycoconjugates; IMR-32 cells; ricin b; neuroblastoma cells

    • Abstract


      Multiple forms of ricin have been isolated from castor bean seeds. Two forms, ricin-1 and ricin-2, differ in their isoelectric pI values and toxicity towards IMR-32 cells. Inhibition of IMR-32 DNA polymerase α2 is more pronounced with ricin-1 (65%) than with ricin-2 (10%). Ricin B chain (pI = 5.2) isolated from ricin-1 binds to IMR-32 cell surfaces as well as inhibits DNA polymerase α2 activity when studiedin vitro. The presence of galβ-linked glycoconjugates near the active site of IMR-32 DNA polymerase α2 has been proposed. Replication modulators which bind to the glycose portion of the enzymes involved in the replication system may need a mandatory binding to cell surface glycoconjugates for their activity.

    • Author Affiliations


      Subhash Basu1 Prabir Bhattacharya1 Manju Basu1

      1. Department of Chemistry, Biochemistry, Biophysics and Molecular Biology Program, University of Notre Dame, Notre Dame, Indiana - 46556, USA
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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