• Towards a subunit influenza vaccine prepared by affinity chromatography on immobilized lectin

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      https://www.ias.ac.in/article/fulltext/jbsc/005/S1/0149-0155

    • Keywords

       

      Influenza subunit vaccine; haemagglutinin; neuraminidase; lectin; Crotalaria juncea ; affinity chromatography; adjuvant

    • Abstract

       

      Disintegration of influenza virions with 0.2% w/v sodium deoxycholate releases haemagglutinin and neuraminidase, which in the presence of detergent are both adsorbed to the lectin fromCrotalaria juncea, coupled to Sepharose 2B.

      The binding is mediated through galactosyl residues on haemagglutinin and neural-minidase, which are completely displaced in 0.2 M lactose and co-elute in a narrow zone.

      The immunogenicity of haemagglutinin and neuraminidase in mice is markedly increased after adsorption onto lipid, particles constituting “intralipid” (Kabi Vitrum, Stocholm, Sweden).

    • Author Affiliations

       

      T Kristiansen1 M Sparrman1 L Heller1 2

      1. Institute of Biochemistry, Biomedical Center, University of Uppsala, Uppsala, Sweden
      2. National Bacteriological Laboratory, Stockholm, Sweden
    • Dates

       
  • Journal of Biosciences | News

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