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Lima bean; lectin; metal ions; lymphocytes; mitogenesis

Two lectins, a tetramer designated LBL₄ and an octamer LBL₈ designated have been purified from the lima beanPhaseolus lunatus. The tetramer appears to be nonmitogenic for human lymphocytes and is a weak mitogen for bovine cells. The octamer and a chemically cross-linked form of the tetramer are good mitogens. The lima bean lectin binds to only certain sub-populations of human lymphocytes. The primary class which does not bind appears to be a sub-population ofT-lymphocytes. Comparisons of cell binding with other lectins which bind to 2-acetamido-2-deoxy-D-galactose have been carried out. Quantitative analysis of the binding to human erythrocytes is co-operative but binding to lymphocytes is non-co-operative. These results show that there may not be a direct correlation between mitogenic stimulation and cooperative binding to membrane receptors.

J A Magnuson¹ E R Pandolfino¹ G R Munske¹ A E Namen¹ M S Nissen¹

1. Biochemistry/Biophysics Program Department of Chemistry, Washington State University, Pullman, Washington - 99164, USA

Published

December 1983