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      https://www.ias.ac.in/article/fulltext/jbsc/005/S1/0061-0064

    • Keywords

       

      Concanavalin A; chromatography; brain hydrolases

    • Abstract

       

      Concanavalin A bound to Sepharose has been used for the purification of brain β-galactosidase, α-L-fucosidase, α-D-mannosidase, arylsulphatase and β-glucuronidase.0 Several factorsviz pH, temperature and concentration of α-methyl glucoside influenced the binding and elution of these enzymes. A lysosomal acid α-mannosidase and a cytosolic neutral mannosidase were separable by concanavalin A-Sepharose chromatography. Similarly lysosomal and microsomal β-glucuronidases were separable using gradient elution with α-methyl glucoside. The results indicate the usefulness of this lectin for the isolation of wide variety of enzymes under specified experimental conditions.

    • Author Affiliations

       

      A S Balasubramanian1 T Alam1 R Mathur1 S Lakshmi1 R Cherian1 K Alvares1

      1. Neurochemistry Laboratory, Department of Neurological Sciences, Christian Medical College Hospital, Vellore - 632 004
    • Dates

       
  • Journal of Biosciences | News

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