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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/005/S1/0031-0039

    • Keywords

       

      Fluorescence polarization; lectin; sugar binding

    • Abstract

       

      The binding ofRicinus communis agglutinin andAbrus agglutinin to 4-methylumbelliferyl β-D-galactopyranoside was studied by equilibrium dialysis, fluo-rescence quenching and fluorescence polarization. The number of binding sites and the association constant value obtained by fluorescence polarization for bothRicinus communis agglutinin andAbrus agglutinin are in close agreement with those obtained by the other methods. This indicates the potential of ligand-fluorescence polarization measurements in the investigation of lectin-sugar interactions.

    • Author Affiliations

       

      M Islam Khan1 M K Mathew1 T Mazumdar1 2 D Pain1 2 N Surolia1 3 M K Das1 2 P Balaram1 A Surolia1

      1. Molecular Biophysics Unit, Indian Institute of Science, Bangalore - 560 012
      2. Department of Biochemistry Indian Institute of Science, Bangalore - 560 012
      3. Enzyme Engineering Laboratory, Indian Institute of Chemical Biology, Jadavpur, Calcutta - 700 032

    • Dates

       
      Published
      December 1983

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

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