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      https://www.ias.ac.in/article/fulltext/jbsc/005/S1/0031-0039

    • Keywords

       

      Fluorescence polarization; lectin; sugar binding

    • Abstract

       

      The binding ofRicinus communis agglutinin andAbrus agglutinin to 4-methylumbelliferyl β-D-galactopyranoside was studied by equilibrium dialysis, fluo-rescence quenching and fluorescence polarization. The number of binding sites and the association constant value obtained by fluorescence polarization for bothRicinus communis agglutinin andAbrus agglutinin are in close agreement with those obtained by the other methods. This indicates the potential of ligand-fluorescence polarization measurements in the investigation of lectin-sugar interactions.

    • Author Affiliations

       

      M Islam Khan1 M K Mathew1 T Mazumdar1 2 D Pain1 2 N Surolia1 3 M K Das1 2 P Balaram1 A Surolia1

      1. Molecular Biophysics Unit, Indian Institute of Science, Bangalore - 560 012
      2. Department of Biochemistry Indian Institute of Science, Bangalore - 560 012
      3. Enzyme Engineering Laboratory, Indian Institute of Chemical Biology, Jadavpur, Calcutta - 700 032
    • Dates

       
  • Journal of Biosciences | News

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