• Human erythrocyte specific lectin from the seeds of Indian coral tree,Erythrina variegata Linn, var.orientali Linn, Merrill

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      https://www.ias.ac.in/article/fulltext/jbsc/005/S1/0025-0030

    • Keywords

       

      Erythrina variegata ; lectin purification; human erythrocyte specific; mitogen

    • Abstract

       

      Human erythrocyte specific lectin was isolated from the seeds ofErythrina variegata Linn. var.orientalis Linn. Merrill. The lectin preferentially agglutinated erythrocytes in the sequence of O>B>A = AB. The lectin was purified 19-fold by affinity chromatography on acid treated sepharose 4B with an yield of 81%. The purified lectin was found homogeneous on polyacrylamide gel electrophoresis. The erythroagglutination reaction was inhibited by N-acetyl-D-galactosamine, D-galactose and lactose at very low concentration. The haemagglutination by the purified lectin was not inhibited by different hexose and pentose sugars even at high concentration. The purified lectin was a glycoprotein and agglutinated leucocytes at 3 μg protein concentration. The lectin induced transformation of peripheral blood lymphocytes in cultures.

    • Author Affiliations

       

      T K Datta1 P S Basu1

      1. Process Biochemistry Department, Indian Institute of Chemical Biology, Calcutta - 700 032
    • Dates

       
  • Journal of Biosciences | News

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