• Human erythrocyte specific lectin from the seeds of Indian coral tree,Erythrina variegata Linn, var.orientali Linn, Merrill

    • Fulltext


        Click here to view fulltext PDF

      Permanent link:

    • Keywords


      Erythrina variegata ; lectin purification; human erythrocyte specific; mitogen

    • Abstract


      Human erythrocyte specific lectin was isolated from the seeds ofErythrina variegata Linn. var.orientalis Linn. Merrill. The lectin preferentially agglutinated erythrocytes in the sequence of O>B>A = AB. The lectin was purified 19-fold by affinity chromatography on acid treated sepharose 4B with an yield of 81%. The purified lectin was found homogeneous on polyacrylamide gel electrophoresis. The erythroagglutination reaction was inhibited by N-acetyl-D-galactosamine, D-galactose and lactose at very low concentration. The haemagglutination by the purified lectin was not inhibited by different hexose and pentose sugars even at high concentration. The purified lectin was a glycoprotein and agglutinated leucocytes at 3 μg protein concentration. The lectin induced transformation of peripheral blood lymphocytes in cultures.

    • Author Affiliations


      T K Datta1 P S Basu1

      1. Process Biochemistry Department, Indian Institute of Chemical Biology, Calcutta - 700 032
    • Dates

  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.