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      https://www.ias.ac.in/article/fulltext/jbsc/005/S1/0009-0017

    • Keywords

       

      Concanavalin A; metal ions; lectin; saccharide binding

    • Abstract

       

      Metal ion activation of saccharide binding has been studied for concana-valin A near pH 7.0. Although two metal ions, a transition metal ion and a Ca2+ ion, can bind, both are not required. Ca2+ alone, Mn2+ alone, or Ca2+ with other transition metal ions can activate this lectin. Only one Ca2+ ion per subunit or only one Mn2+ per subunit is sufficient. Metal ion binding was studied by magnetic resonance techniques and direct binding assays. Saccharide binding activity was monitored by following the fluorescence of 4-methylumbelliferyl a-D-mannopyranoside. When Ca2+ binds to demetalized concanavalin A, the transition metal ion site is hindered. When Mn2+ alone binds to demetalized concanavalin A, saccharide binding activity is induced. A subsequent conformational change, not necessary for carbohydrate binding activity, covers the Mn2+.

    • Author Affiliations

       

      J A Magnuson1 G M Alter1 D M Appel1 D J Christie1 G R Munske1 E R Pandolfino1

      1. Biochemistry/Biophysics Program, Department of Chemistry, Washington State University, Pullman, Washington - 99164, USA
    • Dates

       
  • Journal of Biosciences | News

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