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    • Keywords


      Sulphate incorporation; pituitary hormone; lutropin; gonadotropin; immunoprecipitation

    • Abstract


      The presence of sulphate in the carbohydrate of pituitary lutropin from different species has been investigated using a biosynthetic approach. Pituitaries from rats, rabbits, goats, and buffaloes were incubated in the presence of35SO4- and the35SO4- -labelled proteins in the tissue immunoprecipitated with a well characterized anti-sheep lutropin serum. The incorporation into immunoreactive lutropin was low in the case of rat, rabbit and goat pituitaries while, it was considerable in the case of buffalo pituitaries. Hence further characterization studies were carried out on35SO4- -labelled proteins of buffaloes. The physico-chemical, immunological and biological properties of radio-labelled buffalo pituitary material were shown to be similar to those of standard lutropin. Inin vitro conditions of incubations, most of the incorporation of35SO4- was observed into tissue lutropin while under similar conditions of incubation, [14C]-amino acids were found to get incorporated mostly into medium lutropin. The physiologically specific releasing hormone, lutropin-releasing hormone was found to stimulate the release of35SO4--labelled lutropin from the rabbit pituitaries into the medium. These results give indirect evidence that sulphate could be present in pituitary lutropin.

    • Author Affiliations


      A S Arunasmithasri1 T Rajendrakumar1 K Muralidhar1 2

      1. School of Life Sciences, University of Hyderabad, Hyderabad - 500 134
      2. Department of Zoology, University of Delhi, Delhi - 110 007
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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