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Nicotiana sanderae ; RNA polymerase; phytohormones; differentiation

Isolated nuclei from differentiating cultures ofNicotiana sanderae showed increased levels of RNA polymerase activity as compared to the nuclei from callus cultures. The RNA synthetic activity was dependent on nucleotide triphosphates and Mg²⁺ and was destroyed by RNase. Maximum activity was obtained in the presence of 50 mM (NH₄)₂ SO₄ and α-amanitin inhibited 40% and 55% of the activity in the nuclei from callus and differentiating tissue respectively. The nuclei from differentiating tissue elicited a 3-fold increase in RNA polymerase I and a 4-fold augmentation in RNA polymerase II activities.

P K Asha¹ M S Shaila¹ C S Vaidyanathan^{1 2} T Ramakrishnan¹

1. Microbiology and Cell Biology Laboratory, Indian Institute of Science, Bangalore - 560 012
2. Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012

Published

December 1983

Manuscript received

18 August 1983

Manuscript revised

5 October 1983