• A new DNA-cloning vector forHaemophilus influenzae Rd.

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      https://www.ias.ac.in/article/fulltext/jbsc/005/04/0339-0345

    • Keywords

       

      Cloning vector; EcoRI site; efficient insert detector

    • Abstract

       

      A new, high-efficiency, DNA-cloning vector pJ1-8 was derived in two steps from the chimeric plasmid pD7 consisting of RSF 0885(ampr) andHaemophilus influenzae chromosomal DNA. pJl-8 has only oneEcoRI site and a molecular weight of only 2.5 × 106. No detectableampr transformation was obtained with pJl-8 DNA. However,ampr transformation increases markedly ifHaemophilus influenzae chromosomal DNA segments are spliced into it, providing a very facile assay for detecting inserts.

    • Author Affiliations

       

      V P Joshi1 N K Notani1

      1. Biology and Agriculture Division, Bhabha Atomic Research Centre, Trombay, Bombay - 400 085
    • Dates

       
  • Journal of Biosciences | News

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      Posted on July 25, 2019

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