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    • Keywords


      Ontogeny; insulin receptor; serum insulin; hepatocytes

    • Abstract


      Interaction of [125I]-insulin with intact hepatocytes and its correlation with circulatory insulin level was examined. The hepatocytes from new-born rats bound lowest amount of [125I]-insulin (1.39±0.41 pM/mg cell protein) when circulatory insulin level was high (8±1.5 ΜU/ml). Hepatocytes from 7 day and 21 day old animals demonstrated a more or less similar relationship, Cells from 31 day old animals exhibited maximum insulin binding, activity (5.13±0.18.pM/mg cell protein) against a low serum insulin level (4.25±0.25 ΜU/ ml). Scatchard analysis of insulin binding shows that the affinity is higher in the hepatocytes from new-born animals than in the hepatocytes of 31 day old animals. Higher binding observed in the latter case may be due to a greater number of binding sites. Hepatocytes from one year old rats bound very little insulin (2.50±0.36 pM/mg cell protein) against a high circulatory insulin level (9.25±0.85 ΜU/ml). In view of these results, it appears that the down-regulation hypothesis holds true during ontogeny too.

    • Author Affiliations


      Anil K Balapure1 A K Rastogi1 2 M K Sahib1

      1. Division of Biochemistry, Central Drug Research Institute, Lucknow - 226 001
      2. Division of Biophysics, Central Drug Research Institute, Lucknow - 226 001
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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