• Regulation of phosphoglycerate mutase in developing forespores and dormant spores ofBacillus megaterium by thein vivo levels of phosphoglycerate mutase inhibitor

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    • Keywords


      Bacillus megaterium ; 3-phosphoglyceric acid; phosphoglycerate mutase; mutase inhibitor; dipicolinic acid

    • Abstract


      Bacillus megaterium accumulated 3-phosphoglycerate during sporulation which was utilized during spore germination. During sporulation a protein was synthesized before or at the start of 3-phosphoglycerate accumulation inside the developing spores about 1.5 h before dipicolinic acid accumulation. This protein has an affinity for Mn2+ and other divalent metal ions and inhibits phosphoglycerate mutase activity which has been shown to require Mn2+ However, the levels of the inhibitor decreased considerably (75–85%) during spore germination. No appreciable amount of the inhibitor was detected in the vegetable cell and mother cell compartment; however, the forespore compartment possesses an activity comparable to that of dormant spores. The partially purified inhibitor has a molecular weight of 11,000 and possesses both high and low affinity binding sites for Mn2+ and Ca2+ as determined by Scatchard plot analysis.

    • Author Affiliations


      Ravendra Pal Singh1 2

      1. Department of Biochemistry, University of Connecticut, Health Center, Farmington, Conn. - 06032, USA
      2. Biochemistry Division, Regional Research Laboratory, Jorhat - 785 006
    • Dates

  • Journal of Biosciences | News

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