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      https://www.ias.ac.in/article/fulltext/jbsc/004/04/0413-0418

    • Keywords

       

      Proteoglycan endocytosis; lysosomes; reduced degradation

    • Abstract

       

      Temperature up to 16‡C reduced endocytosis of [35S]-proteoglycans by human skin fibroblasts to less than 15% of that at 37‡C. At temperatures between 20–26‡C endocytosis was more than 50%. At temperatures below 26‡C, the relative rate of degradation of endocytosed [35S]-proteoglycans was several fold less than the rate of endocytosis.

      Codistribution of endocytosed [35S]-proteoglycans and the lysosomal marker enzyme Β-hexosaminidase upon subcellular fractionation indicated that endocytotic vesicles containing [35S]-proteoglycans had fused with lysosomes at 37‡C and at 16‡C. The prolonged halflives of endocytosed [35S]-proteoglycans at 16–26‡C could not be explained merely by a temperature dependent reduction of catalytic activity of lysosomal enzymes participating in the degradation of sulphated proteoglycans.

    • Author Affiliations

       

      Perumana R Sudhakaran1 2 Roswitha Prinz1 Kurt Von Figura1

      1. Institute of Arteriosclerosis Research and Institute of Physiological Chemistry, University of Munster, Federal Republic of Germany
      2. Department of Biochemistry, University of Kerala, Trivandrum - 695 034
    • Dates

       
  • Journal of Biosciences | News

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