• Studies on the conformation of α-globulin fromSesamum indicum L. in cationic and nonionic detergents

    • Fulltext


        Click here to view fulltext PDF

      Permanent link:

    • Keywords


      Circular dichroism; α-globulin; cationic detergent; nonionic detergent; dissociation and denaturation; I-Β and II-Β structures

    • Abstract


      The circular dichroic spectra of α-globulin fromSesamum indicum L. was recorded in the presence of cetyltrimethyl ammonium bromide, Triton X-100 and Brij-36T. The protein in 0.2 M phosphate buffer pH 7.4 had about 25% Β-structure and 5% α-helix, the rest being aperiodic or irregular structure and a-helix, structure was increased by cationic detergent cetyl trimethyl ammonium bromide. But, the increase in α-helix content was much less than that induced by an anionic detergent, sodium dodecyl sulphate. In non-ionic detergent like Brij-36T and Triton X-100, specific Β-structures like II-Β and I-Β were formed along with changes in α-helical and aperiodic structures. These results suggest that the protein has a fairly labile quaternary structure.

    • Author Affiliations


      V Prakash1 P K Nandi1 2

      1. Protein Technology Discipline, Central Food Technological Research Institute, Mysore - 570 013
      2. National Institute of Arthritis Metabolism and Digestive Diseases, National Institute of Health, Bethesda, Maryland - 20014, USA
    • Dates

  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.